Coding

Part:BBa_K1336001:Design

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)


Azoreductase 1B6 (Heat-stable Azoreductase mutant)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 89
    Illegal PstI site found at 170
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 89
    Illegal PstI site found at 170
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 89
    Illegal PstI site found at 170
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 89
    Illegal PstI site found at 170
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 367
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing PCR primers to change the AzoR gene into the BioBrick format, we also had to design primers for site-directed mutagenesis, as there are 2 illegal PstI restriction sites within the native gene (at positions 89 and 170).


Source

From genomic sequence of Pseudomonas putida; AzoR gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.

References